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phosphorylated p protein kinase b  (Proteintech)


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    Proteintech phosphorylated p protein kinase b
    Phosphorylated P Protein Kinase B, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 440 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phosphorylated p protein kinase b/product/Proteintech
    Average 96 stars, based on 440 article reviews
    phosphorylated p protein kinase b - by Bioz Stars, 2026-02
    96/100 stars

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    EIF3B interacts with METTL3 and activates EGFR/AKT signaling in cervical cancer cells. (A) Representative western blots of co-immunoprecipitation assay. (B) Effect of EIF3B on EGFR mRNA expression in HeLa and SiHa cell lines. (C and D) Effects of EIF3B on EGFR protein and p-AKT expression. (C) Representative western blots and (D) quantification of EGFR protein and p-AKT expression in HeLa and SiHa cell lines. EIF3B, eukaryotic translation initiation factor 3B; METTL3, methyltransferase-like 3; p-, phosphorylated; si-, small interfering; oe-, overexpressing; NC, negative control. *P<0.05, **P<0.01 and ***P<0.001.

    Journal: Oncology Reports

    Article Title: EIF3B-METTL3 complex promotes cell proliferation, invasion and EGFR/AKT signaling in cervical cancer

    doi: 10.3892/or.2025.8936

    Figure Lengend Snippet: EIF3B interacts with METTL3 and activates EGFR/AKT signaling in cervical cancer cells. (A) Representative western blots of co-immunoprecipitation assay. (B) Effect of EIF3B on EGFR mRNA expression in HeLa and SiHa cell lines. (C and D) Effects of EIF3B on EGFR protein and p-AKT expression. (C) Representative western blots and (D) quantification of EGFR protein and p-AKT expression in HeLa and SiHa cell lines. EIF3B, eukaryotic translation initiation factor 3B; METTL3, methyltransferase-like 3; p-, phosphorylated; si-, small interfering; oe-, overexpressing; NC, negative control. *P<0.05, **P<0.01 and ***P<0.001.

    Article Snippet: The primary antibodies including EIF3B (1:5,000; cat. no. 10319-1-AP), METTL3 (1:10,000; cat. no. 15073-1-AP), N-cadherin (1:5,000; cat. no. 22018-1-AP), E-cadherin (1:5,000; cat. no. 20874-1-AP), Vimentin (1:20,000; cat. no. 10366-1-AP), EGFR (1:10,000; cat. no. 18986-1-AP), protein kinase B (AKT; 1:10,000; cat. no. 10176-2-AP), phosphorylated (p)-AKT (1:10,000; cat. no. 28731-1-AP) and GAPDH (1:10,000; cat. no. 10494-1-AP; all from Proteintech Group, Inc.) were incubated with the membrane overnight at 4°C.

    Techniques: Western Blot, Co-Immunoprecipitation Assay, Expressing, Negative Control

    EIF3B-METTL3 complex promotes EGFR/AKT signaling in cervical cancer cells. (A) Effect of the EIF3B-METTL3 complex on EGFR mRNA expression in HeLa and SiHa cell lines. (B and C) Effect of EIF3B-METTL3 complex on EGFR protein and p-AKT expression. (B) Representative western blots and (C) quantification of EGFR protein and p-AKT expression in HeLa and SiHa cell lines. EIF3B, eukaryotic translation initiation factor 3B; METTL3, methyltransferase-like 3; p-, phosphorylated; si-, small interfering; oe-, overexpressing; NC, negative control; ns, not significant. *P<0.05, **P<0.01 and ***P<0.001.

    Journal: Oncology Reports

    Article Title: EIF3B-METTL3 complex promotes cell proliferation, invasion and EGFR/AKT signaling in cervical cancer

    doi: 10.3892/or.2025.8936

    Figure Lengend Snippet: EIF3B-METTL3 complex promotes EGFR/AKT signaling in cervical cancer cells. (A) Effect of the EIF3B-METTL3 complex on EGFR mRNA expression in HeLa and SiHa cell lines. (B and C) Effect of EIF3B-METTL3 complex on EGFR protein and p-AKT expression. (B) Representative western blots and (C) quantification of EGFR protein and p-AKT expression in HeLa and SiHa cell lines. EIF3B, eukaryotic translation initiation factor 3B; METTL3, methyltransferase-like 3; p-, phosphorylated; si-, small interfering; oe-, overexpressing; NC, negative control; ns, not significant. *P<0.05, **P<0.01 and ***P<0.001.

    Article Snippet: The primary antibodies including EIF3B (1:5,000; cat. no. 10319-1-AP), METTL3 (1:10,000; cat. no. 15073-1-AP), N-cadherin (1:5,000; cat. no. 22018-1-AP), E-cadherin (1:5,000; cat. no. 20874-1-AP), Vimentin (1:20,000; cat. no. 10366-1-AP), EGFR (1:10,000; cat. no. 18986-1-AP), protein kinase B (AKT; 1:10,000; cat. no. 10176-2-AP), phosphorylated (p)-AKT (1:10,000; cat. no. 28731-1-AP) and GAPDH (1:10,000; cat. no. 10494-1-AP; all from Proteintech Group, Inc.) were incubated with the membrane overnight at 4°C.

    Techniques: Expressing, Western Blot, Negative Control